Nermeen M Arafa
National Research Centre, Egypt
Title: New approach for production of volatile and acephenanthrylene constituents using biotic and abiotic elicitors in cell cultures of Pimpinella anisum L.
Biography
Biography: Nermeen M Arafa
Abstract
Pimpinella anisum L. (anise) is a worthy aromatic medicinal plant involving medical and pharmacological potentials. Our study aimed to apply the biotechnological techniques using biotic (yeast) and abiotic (L. phenylalanine)
elicitors for enhancement anise callus productivity for enrichment their content of bioactivity phytocomponents to be applicable in medical and pharmacological purposes. Total 15 of volatile constituents and acephenanthrylene skeleton were detected comparatively in callus cultures of Pimpinella anisum L. and were identifi ed by GC-MS. Suppression of breast cancer cell lines (MCF7) proliferation was evaluated. Leaf, stem and shoot tip explants of in vitro seedlings initiated calli, shoot tip was a preferable explant on medium containing 1mg/l IAA+1mg/l 2.4-D+2mg/l kin. Fresh weight, growth rate and increase value of shoot tip calli recorded maximum values (18.356±0.269, 2.407±0.038, and 11.237±0.179, respectively) aft er four weeks. Shoot tip calli were improved in treatments of yeast (0.5, 1.0, 1.5 g/l) and 2mM/l L. phenylalanine which increased callus fresh weight to be 17.556±0.195, 21.864±0.428, 26.92±0.440 and 13.734±0.199, respectively compared to the control (12.996±0.284). Total volatile constituents content were boosted to be 32.38%, 40.96%, 43.51% in treatments of 2, 4, 6 mM/l L. phenylalanine, respectively and 45.45%, 31.09%, 26.01% in treatments of 0.5, 1.0, 1.5 g/l yeast, respectively, in comparison to the control (16.83%). 1,2-Diphenyl-5-(tbutyl) acephenanthrylene was detected in all treatments at diff erent proportions. Cytotoxic activity of callus extracts was between IC50 of 1.33 μg/ml to IC50 of 2.39 μg/ml. From the phytocomponents point of view, the percents of acephenanthrylene and volatile constituents in anise callus contributed to their anticancer infl uence.