Alicja Hryniszyn
Title: Advantages and disadvantages of modern methods for the viruses detection in potable water and water for agricultural purposes
Biography
Biography: Alicja Hryniszyn
Abstract
Numerous pathogens are able to survive in environment even for a long time, and then can attack human or other organisms. The permissible concentrations of viruses in drinking water and in water for agricultural purposes have been determined in many directives. However, it is very difficult to evaluate the risk of infection by various viruses including enteroviruses. For the risk assessment, knowledge concerning the pathogen concentration that cause adversely affect on human health is important. However, obtaining data concerning minimal dose of the enteroviruses which cause human diseases is practically impossible (ethical objections, high costs). For that reason, it should be found that any concentration level of viruses present in drinking water or water used for agricultural purposes might be hazardous; thus, their presence in water should be monitored. There are a lot of modern methods which are used for detection of viruses in water. These methods include ViriChip technique (AFM coupled with protein microarray), flow cytometry, electrophoresis, and biosensors. All of these methods have shortcomings, except biosensors. For example, sensitivity of ViriChip technique depends mainly on viral size, and concentration and specific activity of antigens. Flow cytometry requires dissolution of samples and it is not suitable for RNA viruses detection. Biosensors are the future of viral detection in variety of water samples enabling development of studies on more effective methods of viruses elimination.