Asokan C
Sokoto State University, Nigeria
Title: Encapsulation of hybridoma cell culture for the higher secretion of anti-A blood grouping mAbs
Biography
Biography: Asokan C
Abstract
Biopolymer membrane was prepared by using oppositely charged natural biopolymer as a polymer beads. The biopolymer membrane was used for the encapsulation of three hybridoma cell lines to produce monoclonal antibodies. The morphologies of beads and cells growing were observed in inverted microscope. Cell viability was measured by trypan blue dye exclusion method using a haemocytometer. Theincreased secretion of monoclonal antibody is confirmed by the antibody by titre analysis and protein estimation. These results indicate the effectiveness of encapsulation for hybridoma cell culture. The present study concludes that the yield of diagnostic antibodies is maximized by the encapsulation method.
