Reza Jalilzadeh Yengejeh
Title: The Biodegradation of Crude Oil by Spices of Bacillus Isolated From Contaminated Soil
Biography
Biography: Reza Jalilzadeh Yengejeh
Abstract
In this study, biodegrading ability of spices of isolated bacteria from the soil which was contaminated by Crude Oil was studied. Soil samples were taken from different areas of contaminated soil by Crude Oil. More than 10 important species were identified by culture and biochemical test (Preliminary identification) and molecular tests including sequence determination analysis by polymerase chain reaction (PCR) and 16S rRNA (final identification). Isolated species (specially bacillus) are able to biodegrade Crude Oil Carbohydrates by producing Bio-Surfactants and lowering the rate of Surface Tension at different temperatures and pHs. The decrease of surface tension from 57mN/m to 31mN/m at 37 °Cand to 32.5mN/m at 20 °C by Bacillus Cereus and57mN/m to 30.5mN/m at 37 °Cand to 31.5mN/m at 20 °C by Bacillus subtilis in samples with 1% Crude Oil can be referred to as the results of this research.