Araceli Olivares
Pontifical Catholic University of Valparaiso, Chile
Title: Effect of simulated gastric juice over viability in microencapsulated Lactobacillus casei
Biography
Biography: Araceli Olivares
Abstract
Probiotics must be delivered alive to exert a positive health effects in site of action (the intestine). Once reach the intestine, they should establish themselves and exert a positive health effects. They must be survive the stomach acidic, bile acid and others degradative enzime througth intestinal tract.
The aim of current study was to evaluate the performace of microencapsulated Lactobacillus casei using alginate gel matrix to facilitate a suitable carrier system to enhance this objetive and confer protection from the acidity.
A solution with lyophilized L. casei at 5g/L (108 CFU/mL) was prepared and mixed (1:1) with sodium alginate solution at 2%. As a hardening solution, calcium chloride at 0.1M with gently stirring was used. Microencapsulator provide by BÜCHI (Encapsulated B-390) was used.
Simulated gastric juice (SGJ) with 9g/L of sodium chloride and 3g/L of pepsin was prepared. Different pH was adjusted with 1M HCl. 400mg of microspheres containing L. casei were mixed in 20mL of SGJ with pH adjusted at 2.0; 2.5; 3.0; 3.5 and 4.0 and incubated until 120 min a 37oC and 50rpm. Microencapsulated Lactobacillus were filtered and dissolved in sodium citrate to released and count CFU in supernatant.
The best results were obtained at pH 4.0 (over 70% of survival at 90min). At pH 3.5 the survival was over 20% at 60 min, however at pH less than 3.0 there was a rapid loss of viability and the survival was null at 90 min. Then, a microencapsulated is an available technique to maintain viability of probiotics to protect the passage through intestinal tract.
Acknowledgment: Project Fondecyt No 11130460