13th Biotechnology Congress

Biography

Biography: Wook Jin Kim

Abstract

GennusPaeonia is an important medicinal plant in Asian traditional medicines. Among Paeonia species, P. lactiflora, P. japonica, P. veitchii, and P. suffruiticosa are pharmaceutically defined in different ways in the national pharmacopieias in Korea, Japan, and China.The roots of three Paeonia species, P. lactiflora, P. japonica andP. veitchii, commonly has been used as Paeoniae Radix, and the root bark of P. suffruticosahas been used as MoutanRadicis Cortex in Korean Traditional Herbal Medicine. However, only the roots of P. lactifolora and root bark of P. suffruticosa is pharmaceutically described as Paeoniae Radix and MoutanRadicis Cortex, respectively, in the pharmacopoeia of China and Japan.Since the morphological similarities of root and aerial part of these species, the identification of accurate species is very difficult. In addition, these herbal medicines bistributed as dried root slices or processed medicinal ingredients in the herbal market. Therefore, it is important to authenticate the different species used in these herbal medicines. So, we analyzed DNA barcode seqeunce of rDNA-ITS regionusing 17 samples of four Paeoniaspecies, and thenobtained species-specific marker nucleotides that can be used as genetic markers to identify these four plants at the species levels.Based on rDNA-ITS sequences, peptide nucleic acids (PNA) analysis which of probe-based fluorescence melting curve analysis was carried out to develop a powerful technique for detecting species-specific point mutations, namely marker nucleotide or single nucleotide polymorphism, capable to discriminate the fourPaeonia species without sequence analysis. Also, this method can provide rapid and efficient authentication of four Paeoniaspecies. Therefore, PNA analysis of four herbaciousPaeonia species will help to accurately authenticate each species and standardize the origin and quality of Paeoniae Radix and MoutanRadicis Cortex.