Kamna Srivastava
University of Delhi, India
Title: Renin-angiotensin-aldosterone system genomics in essential hypertension
Biography
Biography: Kamna Srivastava
Abstract
Objectives: Hypertension is one of the major cardiovascular diseases. Candidate genes encoding the Renin-angiotensin-aldosterone system (RAAS), ie, Angiotensin Converting enzyme (ACE), Angiotensinogen, Angiotensin II Type I receptor, Atrial natriuretic peptide (ANP) and Aldosterone synthase (CYP11B2), their expression at genetic and protein levels and their association with essential hypertension, if any, were investigated in a Northern Indian population.
Methods: Genotyping and Gene expression at mRNA and protein levels was carried out by PCR-RFLP, Real time PCR and Western blot respectively.
Results: A significant association was found in the AT1R genotypes (AC+CC) with essential hypertension. The expression of angiotensinogen was also upregulated in patients as compared to controls. The decreased levels of ANP gene expression at mRNA (85%) and protein (72.6%) levels and increased in CYP11B2 protein expression (1.53 fold) in the patient group as compared to controls were found. The individuals with rare allele in Angiotensinogen gene were found to have significant control in blood pressure with ACE inhibitor, Enalapril.
Conclusion: Our findings suggest the association of candidate gene of RAAS with essential hypertension. The increased expression of Angiotensinogen converting enzyme, Angiotensinogen, Angiotensin II Type I receptor gene and decreased levels of ANP gene expression at mRNA and protein levels in the patient group as compared to controls were significantly associated with essential hypertension and could be served as a prognostic biomarker for essential hypertension.