Dr. Nawab Ali
faculty member in the Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan.
Title: Molecular Characterization and Growth Optimization of Protease Producing Pseudomonas aeruginosa strain (IBC-2)
Biography
Biography: Dr. Nawab Ali
Abstract
Microbial proteolytic enzymes are used to hydrolyze proteins in various bio-industrial applications but most of the enzymes are not active in extreme conditions of temperature, pH and salt concentrations. This increases the demand to explore enzymes to show activity at such extreme environments. Exciting findings have been described about the extremophilic bacteria isolated first time from coal mines of Orakzai Agency, Pakistan, having protease producing properties. Among various extracted isolates, â“6 isolates were purified based on showing proteolytic activity using 2% skim milk (SM) agar media at 37oC for 24 h, to identify various morphological and biochemical characteristics. The isolate, IBC-2 showed the maximum zone of activity (21mm) and was identified as Pseudomonas aeruginosa (JCM 5962) using 16S rRNA gene sequencing. The strain IBC-2 exhibited significantly (P ≤ 0.05) maximum growth and protease production (335 U/ml) at 37°C and at pH 7 after 24 h of incubation. The strain was also significantly (P ≤ 0.05) more active for protease production at neutral pH (435 U/ml) than at high or low pH which reflected the neutral nature of the bacteria. In conclusion, the strain IBC-2 has the highest protease producing ability with distinctiveness, which can