Hong Zhao
Wuhan University, China
Title: 2Gy, 6Gy, 8Gy, 10Gy may be some ideal fractional doses with the better biological response
Biography
Biography: Hong Zhao
Abstract
To study the radiation response of the cancer cells to different fractional dose irradiation and explore the ideal fractional dose. Methods: Observe the variability of cell cycle, cell proliferation, apoptosis, DNA damage and repair and the expression of related proteins of human breast cancer cell line MDA-MB-435 which was administrated by different dose of X-ray irradiation (0Gy, 2Gy, 4Gy, 6Gy, 8Gy, 10Gy, 12.5Gy, 15Gy, 20Gy). Flow cytometry was used to observe the rate of apoptosis and cell cycle arrest, CCK8 method and colony formation assay were used to examine cell proliferation, the expression of DNA damage and repair and other related proteins were detected with western blotting.