Rajesh M. Patel
Ganpat University, India
Title: Apoptic induction activity of methanolic extract of Aristolichia Indica plant against K562 cells
Biography
Biography: Rajesh M. Patel
Abstract
Apoptosis is programmed cell death which occur in multicellular organisms and is characterized by a series of events lead to a variety of morphological and biochemical changes, including membrane blebbing, cell shrinkage, alteration of membrane asymmetry and permeability, condensation of chromatin and nucleus, DNA fragmentation, and formation of membrane bound vesicles (apoptotic bodies). Apoptosis and cell mediated cytotoxicity are characterized by cleavage of the genomic DNA into discrete fragments prior to membrane disintegration. Because DNA cleavage is a hallmark for apoptosis, assays which measure DNA fragmentation are used for the determination of apoptotic cell death. The DNA fragmentation analysis was carried out to determine apoptosis inhibition by test extract if extract might have toxicity followed by cleavage of nuclear DNA. The DNA fragmentation was performed with IC50 dose of the methanolic extract of Aristolochia indica against K562 cancer cells and vero normal cells. From the results, it found that the extract was exhibited significant DNA fragmentation pattern, which confirmed induction of apoptosis rather than necrosis. The 100 bp ladder was used as standard marker. Methanolic extract having concentration of 1000 µg/ml showed the significant DNA fragmentation pattern in K562 cells. While fragmentation pattern was not significant (no clear single band) in vero cells. So it revealed that methanolic extract exhibited indusive effect of apoptosis leads to cell death.