Sadaf Fatima
Jamia Millia Islamia, New Delhi, India
Title: Biological activities of Indian Ayurvedic formulation, Panchakola
Biography
Biography: Sadaf Fatima
Abstract
A large number of studies have proven the efficacy of ayurvedic herbs in the field of health and wellness. The aim of this study was to access the in-vitro biological potential of Indian ayurvedic formulation, Panchakola which is the equi-proportional mixture of five herbs. The aqueous extract of powdered panchakola was characterized for its antioxidant and anti-inflammatory activity. Further, the aqueous extract was analyzed for its antibacterial property against eight bacterial strains including two strains of Escherichia coli, one each of Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Aeromonas species and Klebsiella oxytoca. Further, the aqueous extract of panchakola was tested against Candida cells to reveal its role in antifungal activity. The results revealed that http://www.biotechnologycongress.com/asia-pacific/ showed 50% DPPH scavenging at 123 μl of extract as compared to the Ascorbic acid's value at 25μl which is known to be a strong antioxidant agent. Also, the highest FRAP value was observed at 500 μl and the lowest was at 50μl i.e. 0.989 mM and 0.419 mM as compared to Ascorbic acid i.e. 1.03 mM and 0.201 mM respectively. The panchakola extract showed no anti-inflammatory activity in both the assays upto a maximum amount of 1000 μl. It was found that panchakola had strongest inhibitory activity against E. coli (76.49%), minimal inhibition against P. aeruginosa (28.43%) and no inhibition against B. subtilis. Hence, results suggested that it can be used to treat oxidative stressed disorders and also can replace the antibiotics.