Sarabjeet Singh Ahluwalia
General Shivdev Singh Dewan Gurbachan Singh Khalsa College, India
Title: Decolorization of textile dyes Basic Red 46 and Reactive Black 5 by Staphylococcus epidermidis MTCC 10623
Biography
Biography: Sarabjeet Singh Ahluwalia
Abstract
A critical situation has aroused due to increased discharge of wastewater containing considerable extent of azo dyes from textile dyeing and manufacturing industries, which requires prior treatment, before being released in order to prevent contamination of natural water courses and ground water. In the present study, Staphylococcus epidermidis MTCC 10623 isolated from contaminated site was explored for the decolorization and degradation of two different types of azo dyes such as Basic Red 46 (BR 46) and Reactive Black 5 (RB 5), extensively used in textile processing industries. S. epidermidis has shown remarkable decolorization potential (99.6 and 97%) for the selected dyes at the concentration of 100 mg/L under optimized conditions (pH: 9.0; temp: 40 and 35 °C) after 6 and 24 hours incubation respectively. Moreover, addition of carbon (glucose: 0.3%) and nitrogen (ammonium sulfate: 0.1%) source as co-substrates enhanced the decolorization potential of S. epidermidis. Further, decolorization of BR 46 and RB 5, was achieved 99.7 and 97.2% decolorization within 6 and 24 hours respectively with immobilized S. epidermidis over polyurethane foam. Biodegradation analysis of metabolites formed after bacterial treatment by UV-Vis and FTIR spectrophotometer confirms the degradation of these dyes. Phytotoxicity assay of extracted metabolites on Triticum aestivum and Vigna radiata showed good germination rate and growth of radical and plumule demonstrating the non-toxic nature of treated dye solution and suggest that this bacterium is the efficient azo dye degrading bacteria and has practical application in biological treatment of dye-polluted wastewater streams.