Weizhong Jin
Nanjing Medical University, China
Title: Development of a pH / PO2 dual-parameter fluorosensor
Biography
Biography: Weizhong Jin
Abstract
The accurate measurement of blood pH and PO2 is often critical in the clinical assessment of patients with severe illness. Continuous intra-arterial blood gas and pH monitoring by use of optical sensors offer certain advantages over other types of sensors. Miniaturization is vital for the continuous intravascular blood gas monitoring probes. But the most recent devices had withdrawn from market partly due to the bundled probes. The present study aimed at preparing and evaluating a pH / PO2 dual-parameter fluorosensor by use of a single optic fiber. We fabricated a fiber-optic pH fluorosensor based on a proton-sensitive fluorescent dye AMPN firstly. A PO2-sensitive filming, in which the PO2-sensitive indicator Ru(dpp)3(PF6)2 was immobilized, was coated to the upper section over the pH sending membrane. The fluorescent intensity at 510 nm and 615 nm was recorded separately when the fabricated dual-parameter probe was excited at 395 nm. Its selectivity and accuracy were analyzed when the probe was exposed to the buffers with different pH or bubbled by 100% N2 or O2 in turn. The sensor emitted green and red dual-color fluorescence simultaneously when excited at 395 nm. Both the two fluorescence intensity peaks at 510 nm and 615 nm changed separately with different pH or PO2 values and weren’t almost interfered each other.The pH / PO2 dual-parameter fluorosensor can determine accurately the two signals simultaneously, making the two signals transmitted through a single optic fiber and miniaturizing the multi-parameter sensor.