Jin Ho Lee
Hannam University, South Korea
Title: Dual growth factor-immobilized microspheres for tissue reinnervation
Biography
Biography: Jin Ho Lee
Abstract
Growth factors [basic fibroblast growth factor (bFGF) and/or nerve growth factor (NGF)]-immobilized polycaprolactone (PCL)/Pluronic F127 microspheres were prepared using an isolated particulate-melting method and the sequential binding of heparin and growth factors (GFs) onto the microspheres. The GFs immobilized on the microspheres were released in a sustained manner over 28 days, regardless of GF type. From the in vitro culture of muscle-derived stem cells, it was observed that the NGF-immobilized microspheres induced more neurogenic differentiation than the bFGF-immobilized microspheres, as evidenced by a quantitative real time polymerase chain reaction using specific neurogenic markers (Nestin, GFAP, β-tubulin, and MAP2) and Western blot (markers, Nestin and β-tubulin)] analyses. The dual bFGF/NGF-immobilized microspheres showed better neurogenic differentiation than the microspheres immobilized with single bFGF or NGF. From the preliminary animal study, the dual bFGF/NGF-immobilized microsphere group also showed effective nerve regeneration, as evaluated by immunocytochemistry using a marker, β-tubulin. The dual bFGF/NGF-immobilized PCL/Pluronic F127 microspheres may be a promising candidate for nerve regeneration in certain target tissues (i.e., muscles) leading to sufficient reinnervation [This work was supported by a grant of Korea Ministry of Health & Welfare (HI14C0522)].