Hong Zhao
Wuhan University, China
Title: Glucose metabolism related enzymes may become some ideal targets to increase radiosensitivity of cancer cell
Biography
Biography: Hong Zhao
Abstract
To establish a radioresistant cell line of cancer cells and investigate the proteins related to glucose metabolism, find out the differences between them. Methods: established radioresistant cell line (435R) of breast cancer cell line MDA-MB-435 through exposed to continuous X-ray radiation(2Gy a day for five days a week and a total dose of 60Gy), the radiation resistance of cells was detected by colony formation assay. Enzymes that related to the glucose metabolism of the cells were detected at the level of transcription and translation levels. The changes of the metabolite concentrations were detected with the special kits.