Syarul Nataqain Binti Baharum
Universiti Kebangsaan Malaysia, Malaysia
Title: Metabolomics approach for biomarker discovery of Epinephelus fuscoguttatus infected with vibriosis
Biography
Biography: Syarul Nataqain Binti Baharum
Abstract
Epinephelus fuscoguttatus or tiger grouper has excellent biological characteristics, fast growing and suitable for aquaculture. This posed a promising industry but is unfortunately hindered by a host of diseases, which often associated with feeding leading to great economic loss. Our goal is to study the metabolite responses to vibrosis in grouper and identify unique metabolites as biomarker to help future remediation/prevention of vibriosis. In this work, larvae of grouper were infected with vibriosis, under fed and non-feed conditions. Metabolites were then extracted from infected (challenged) and non-infected (control) larvae, which died and compared with that of survived. Metabolites profiled were obtained using GC-MS based metabolome analysis. The differences between the challenged and control samples were significantly influenced by the production of fatty acids. A total of 11 fatty acids under challenged feeding and 13 fatty acids under challenged starvation of ï·-9 were detected. However, in the control feeding and control starvation conditions, only four fatty acids of ï·-9 were detected namely, lauric acid, myristic acid, palmitic acid and trans-9-palmitoleic acid. The detected compounds were quantified based on three different extraction techniques with the highest at 34 mg/ml and the lowest at 17 mg/ml and mapped in the major fatty acids pathway. There were significant differences (P≤0.001) of ï·-9 production between the challenged and control samples. These changes demonstrate that vibriosis in the fish will induce a biochemical response, especially ï·-9 that can be used as biomarker to determine the survival rate of E. fuscoguttatus.