10^th Asia-PacificBiotech Congress

Biography

Biography: Zurina Zainal Abidin

Abstract

Pathogens that usually associated with pathogenesis affect human health with massive diseases including chronic infections or immune system disorders. Although numerous vaccinations have been introduced to protect against diseases, some pathogens continues to threaten living life. The current pathogen detection method which based on molecular culture and PCR techniques are essentially slow and time consuming. In recent time, several researchers have attempted to develop rapid detecting tool. Despite advanced engineering, there is still need for an accurate and rapid pathogen detection tool. Thus, this research was carried out to highlight on detection of pathogens using optical biosensor. An optical biosensor (microchannel) is relatively an accurate method of early detection of pathogen. Optical microchannel was fabricated with fiber optics by using photolithography method. Fiber optic biosensor is light scattering, absorption and optical properties of the microorganisms. The chemical composition, energy, the total nucleotides and photo pigments will define the absorption properties of each microorganism. E. coli was detected at region of 280 nm to 285 nm by using the microchannel while, S. cerevisiae identified at visible region of 570 nm to 580 nm. The entire detection can be done in less than 10 minutes with minimum required cells of 1x102 cells per mL with total volume of 6 µl. These detection regions for each sample were compared with spectrophotometer measurement plus theoretical calculations by using Beer Lambert law of absorption.