Tingting Deng
Chinese academy of Inspection and Quarantine, China
Title: Quantitative identification of species and GM ingredients in food
Biography
Biography: Tingting Deng
Abstract
GM content in food and feed is strictly regulated in most countries around the world. The implementation of regulation requires
the establishment of GMO detection methods that are specific, reliable, sensitive and suitable for quantitative GMO detection.
Meat adulteration and mislabeling is also a world widely concern in recent years. However, the determination of adulteration ratio
has become a critic issue due to the lack of effective quantitative technique. This presentation will report droplet digital PCR method
for quantitative determination of GMO rice events and meat species in food. The experimental amplification results of relative
quantification all perfectly matched the theoretical results and the limit of quantitative (LOQ) of GMO was determined to be 0.1%,
lower than the labeling threshold level of the EU and many countries, while the meat species method was confirmed to be 1% (w/w).
Moreover, its use for several types of food, feed and seed matrices commonly found in routine samples has been verified.