Khwaja Hossain
Mayville State University, USA
Title: Uptake and translocation of bio-based polymer in plant tissue
Biography
Biography: Khwaja Hossain
Abstract
In recent years interest in bio-based polymers has accelerated because of its renewable and biodegradable properties. However, the
first generation bio-based polymers were mostly derived from agricultural feedstocks which contradict directly with human food
demand. There is limited research in isolating bio-based polymers from crop wastes and their utilization in plant system. Our ultimate
goal is to isolate plant polymer, xylan from wheat bran. After characterization of the polymer using spectroscopic and chromatographic
techniques, chemical functionalization of xylan hydroxyl groups was carried out to form a type of nanoparticles within the size range
of 50-100 nanometer (nm). These particles function as extremely small nanocapsules and will be used to encapsulate micronutrients
for delivering into plant cells. In this preliminary work, to understand the uptake and translocation of bio-based polymer in plant
system, we prepared a Poly (lactic-co-glycolic acid) nanoparticles (NP) tagged with Alexa Fluor 647 dye and applied to germinating
seeds of common bean. Analyzing the polymers treated and non-treated tissue of germinated seeds using confocal microscopy, we
observed differential uptake and translocation of Alexa Fluor 647 dye bound polymer in common bean tissues