K. Yankulov
University of Guelph. Canada
Title: Novel synthetic anti-microbial defensins through confrontational selection and screening of yeast libraries
Biography
Biography: K. Yankulov
Abstract
Antibiotics are frequently used in animal feed to boost its efficacy. However, recent policies in EU and USA have restricted such use of antibiotics and Canada is expected to follow suit. Consequently, the need for novel anti-microbial agents is a most urgent issue. Defensins form a large group of secreted animal, plant or fungal peptides that kill a broad spectrum of microorganisms including food borne pathogens. They have a low probability of developing microbial resistance and are viewed as viable alternatives to antibiotics for both the food and pharmaceutical industries. The industrial development of vertebrate defensins is hindered by concerns of cytotoxicity. In contrast, the known fungal defensins show little side effects in animals and work at doses comparable to these of many antibiotics. We are developing a platform that will use DNA libraries for a huge variety of synthetic defensin-like peptides. We are expressing these libraries in yeast and screen them against model microorganisms. Our aim is to identify novel synthetic anti-microbial peptides that can be used as alternatives to antibiotics. These agents can be improved to incorporate a trypsin-target site to ensure their normal degradation in the stomach and to abolish any side effects on the normal microbiome of the animals. We also aim at the affordable production of such agents by the yeast K. lactis in milk whey. Our progress in these screens will be reported and discussed.