P Anitha
BMS College for Women, India
Title: Germplasm conservation of Andrographis paniculata through somatic embryogenesis and encapsulation
Biography
Biography: P Anitha
Abstract
Andrographis paniculata Nees of the family Acanthaceae, one of the most potential medicinal herbs is used in many pharmacological properties. The main constituent of the herb is Andrographolide; in addition to this Andrographolide esters and lactones have been found to be cancerolytic, hepatoprotective, anti HIV etc., (under testing in vitro). The main objective is to conserve germplasm through somatic embryogenesis and encapsulation. The present investigations have revealed hypocotyls, cotyledons and embryo cultures of Andrographis paniculata were induced to produce somatic embryos on MS media supplemented with 2,4-D (4.56 µm). Somatic embryos at different stages of development were transferred on to maturation and germination media. Somatic embryos were encapsulated and transferred to the recovery media and plantlets were developed. In vitro conservation through somatic embryogenesis and encapsulation may offer a better approach compared to organogenesis for developing scale-up technology by employing bioreactors