Sabrina Schulze
University of Potsdam, Germany
Title: Characterizing constitutive promoters in yeast
Biography
Biography: Sabrina Schulze
Abstract
Design and establishment of new biosynthetic pathways in yeast are important goals in synthetic biology. Therefore, promoters with predictable and reproducible protein expression levels independent of the protein of interest are needed. Data presented here show a library of constitutive promoters expressing two different fluorescent proteins in diverse conditions. We compare expression levels for episomal and chromosomal location, different growth media and different growth times. GFP and RFP are quantified via fluorescence spectroscopy and flow cytometry.