P C Madhu
MET’S School of Engineering, India
Title: Endophytic bacterial L-asparageanase from Simaroubagluaca and study of its anticancer effect
Biography
Biography: P C Madhu
Abstract
Endophytic bacterial L-asparageanase from simaroubagluaca and study of its anticancer eff ect Cancer cells diff erentiate themselves from normal cells in diminished expression of L- asparaginase. It is the enzyme that catalyzes the hydrolysis of L-Asparagine to L- aspartic and ammonia, because of these it is used as a medication and in food manufacturing. L-asparaginase is used to treat various types of leukemia such as, acute lymphoblastic leukemia, acute myeloma leukemia and Non--Hodgkin’s lymphoma. Mostly L-asparaginase is produced and isolated from bacteria from diff erent sources. In the present study we isolated and L-asparaginase producing entophytic bacteria from Simaroubagluaca. Aft er the partial purifi cation of the L-Asparaginase enzyme, the enzyme activity was found to be 155.83 Units/ml and specifi c activity of 770.15. Th e optimum pH is 8 at a temperature of 37oC in the presence of metal ion Mg2+.Th e molecular identifi cation was done by16S rDNA. PCR and sequence analysis by BLAST was performed to identify the similarity and it was confi rmed that Bacillus cereus species. Th e results obtained from the anticancer assay with HL-60 cell lines, up to 95% cell death occurred by incubation with 20 units of enzyme. Th e study reveals that the L-asparaginase produced from Bacillus cereus possess the anticancer activity with HL-60.