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M Cristina Diez

University of La Frontera, Chile

Title: Metagenomic analysis in a bio-purifi cation system used to pesticides degradation


Biography: M Cristina Diez


The biomixture composed by soil, wheat straw and peat, is the main component of the Pesticide Bio-purifi cation Systems (PBS). Previously used biomixture (PUB) from PBS, has microorganisms capable of pesticides biodegradation at high concentrations. In this work, we evaluated the bacterial biodiversity through a metagenomic analysis of a natural biomixture (C) and a biomixture enriched with PUB before and aft er reapplication (60 days) of a mixture of atrazine (ATZ), chlorpyrifos (CHL) and iprodione (IPR) at 100 mg L-1 each. For this, glass deposits (40×25×15 cm), containing
the control biomixture (C) and a biomixture with PUB (B) were used. Metagenomic analysis of B was performed at
day 60 before reapplication of pesticides mixture (B60) and 2 days before pesticides mixture reapplication (B60R). Th e
same analysis was made for control biomixture (C). Microbial diversity was determined by sampling the aforementioned
treatments, from which the DNA was extracted following the manufacturer's instructions (MoBio Powersoil DNA isolation
kit (MoBio, Carlsbad, CA) and quanti-qualitatively characterized by a fl uorometer (double-stranded DNA; dsDNA) and
agarose gel electrophoresis. Metagenomic analysis was performed using MinION sequencer (ONT). Plotts and analysis of taxonomic abundance were made with Pavian 0.3. Regarding all treatments taxonomic composition, treatment B60 showed a greater bacterial abundance in comparision to the other treatments. However, it shows similarity at phylum level with C. On the other hand, the reapplication of pesticides (B60R) decreased momently bacterial abundance and diversity, due to the impact of the mixture of pesticides. However, the biomixture possess a recovering capacity aft er each pesticide