Piyali Das
West Bengal University of Animal and Fishery Sciences, India
Title: Development of animal cartilage for application in human patient
Biography
Biography: Piyali Das
Abstract
The present experiment started with the development of acelullar goat choncal cartilage by glutaraldehyde treatment (0.25% and 1%) for a particular time interval. The soluble protein was extracted and estimated from the native and treated cartilage. The molecular weight was also determined by SDS polyacrylamide gel electrophoresis. Further, delayed type hypersensitivity (DTH) reaction was carried out in rabbits against acelullar and native cartilage protein. In vitro immunocompatibility assay which include lymphocyte proliferation assay and cytotoxicity assay were performed using rabbit peripheral blood mononuclear cells before and after xeno-transplantation with treated cartilage. Detection of antibody by agar gel precipitation test (AGPT) on xeno-trasnplanted rabbits was also assesed at regular time intervals. The in vitro cellular reactivity was found to be less in case of processed cartilage protein than untreated protein and control in both naïve (before xeno-transplantation) and sensitized (after xeno-transplantation) rabbits. Absence of clear precipitin bands in AGPT indicates the absence of specific antibody against ‘treated’ cartilage protein. Absence of any type of DTH reactions after injecting ‘treated’ protein (intradermally) to the animals indicates that the cartilage protein was less reactive. Histological evaluation of treated cartilage sample showed degeneration, loss of chondrocytes and formation of multiple numbers of vacuoles in the cartilaginous matrix as compared to untreated sample where there was presence of fibrocartilagenous structure characterized by formation of well organized chondroblast and chondrocytes. After 3 months of post xeno transplantation in vivo, cartilages along with the surrounding tissues were histochemically studied which involved the qualitative estimation of collagen, reticulin and elastin fibers. It was observed that the orientation of these three elements are properly arranged in the cartilaginous matrix and maintained their normal structure. Based on the result, it can be inferred that this acellular cartilages have tremendous potential for application in human plastic surgery patients.