Biotechnology

Biography

Biography: Suresh Kumar Jatawa

Abstract

Expression profiling of genes that coordinate the mechanisms of DNA repair, cell cycle control, apoptosis and oncogenesis offers a great opportunity for studying multi factor diseases and for understanding the key role of genes in mechanisms which drive a normal cell to a cancer state. Gallbladder cancer (GBC) is an uncommon but highly malignant tumor with varied geographical distribution and poor diagnostic manifestation in early stage. This necessitated a more objective elucidation of GBC at its molecular level. Tissues of (92) patients of gallbladder cancer were examined for gene expression, microsatellite instability and apoptosis through in-house standardized protocols. Resulting data have shown that the women are at high risk for GBC with maximum cases of adenocarcinoma (76%). Highest number of cases has been diagnosed for moderately differentiated adenocarcinoma (68.57%), which evidence lack of proper diagnosis. Mutations of cell cycle regulatory proteins were detected comprehensively in adenocarcinomas (p<0.001). Out of these the expression frequency of cell cycle regulatory genes was higher in moderately differentiated adenocarcinoma in comparison to poorly and well differentiated ones. Microsatellite instability may also be an independent prognostic marker for assessing risk of occurrence in superficial tumors irrespective of the grade. Together these results imply that the isocyanates are potent enough to cause genomic instability under both occupational and accidental exposures along with providing us the impetus for detailed interpretation of the patho-physiological implications of exposure to human beings. We anticipate these data would help us in designing better approaches in risk assessment of future industrial disasters.