Ram Lakhan Singh
Dr. Ram Manohar Lohia Avadh University, India
Title: Microbial decolourization of textile dye orange II by Sphingomonas sp. RMLRK04
Biography
Biography: Ram Lakhan Singh
Abstract
Synthetic dyes are widely used in textile, food, leather, cosmetics and pharmaceutical industries with the textile industry as the largest consumer. The textile finishing generates a large amount of dye containing wastewater that forms one of the largest contributions to water pollution. The removal of the polluting dyes is an important problem particularly for small scale textile industries where working conditions and economic status do not allow them to treat their wastewater before disposal. Biological treatment processes provide an alternative to existing technologies for the decolorization of dyes due to their cost effective and environment-friendly nature. In this study, a bacterial strain Sphingomonas sp. RMLRK04 having capability to decolorize orange II dye was isolated from soil samples collected from the vicinity of textile dyeing units located in Kanpur (India). Studies were conducted to see the effect of various culture and nutritional conditions on decolourization ability of the bacterial strain for orange II dye in minimal salt medium. The optimum pH and temperature for the decolourization of orange II was observed at 7.0 and 30o C respectively during 24 hours of incubation under shaking condition. The decolourization efficiency of this strain using glucose and yeast extract showed increase in rate of decolourization among different carbon and nitrogen source. The bacterial strain showed maximum decolourization at 100 mg l-1 dye concentration and could tolerate up to 100 mg l-1 of dye. The bacterial strain RMLRK04 showed good potential for decolourization of orange II dye.