Rani Gupta
University of Delhi, India
Title: Novel extracellular lipases from Trichosporon asahii MSR54:implications in cell physiology and biotechnology
Biography
Biography: Rani Gupta
Abstract
Three novel lipases TALipA, TALipB and TALipC from opportunistic pathogen Trichosporon asahii MSR54 were isolated, sequenced and heterologously expressed in E.coli and Pichia pastoris X33. Both glycosylated and non-glycosylated proteins were characterized. These lipases are biotechnologically important because all the three lipases are enantioselective. Out of the three, TALipC has an asprich domain and shows enantioinversion in different solvents. It shows Mg activation and calcium inhibition. The role of these lipases was studied in growth and biofilm formation by knock-down strategy. The RNAi silencing and agrobacterium mediated transformation for developing knock-down strains of T. asahii was standardized in our laboratory for the first time. The lipase knock-down strains lost the property of yeast to fungal transition and predominantly retained the yeast stage. Detailed structural and functional comparisons of these lipases will be highlighted.