Biotechnology

Biography

Biography: Rohini R Nair

Abstract

The etiologies of first trimester loss are multifactorial and often remain unknown, certain risk factors increase the likelihood of pregnancy loss. To date there are many factors known to cause EPL but due to very heterogeneous condition of EPL or RPL, it still remains unresolved and unexplained. The identification of the underlying causes of pregnancy loss is important for developing more successful treatments for women experiencing RPL and thereby preventing subsequent pregnancy loss. A multitude of immunomodulatory properties of the fetal-maternal interface have evolved to allow the survival of the immunologically distinct fetus without an attack from the maternal immune system. To identify the contribution of inflammatory molecule we studied the level of S100A8 the inflammatory protein and MDSCs the cells regulating its expression in pregnancy loss patients. The contribution of S100A8 and myeloid-derived suppressor cells (MDSC) in patients suffering from early or recurrent miscarriage is unknown. S100A8 is a calcium binding proteins and is found at high levels in the extracellular milieu during inflammatory conditions. MDSC are implicated in modulation of T-cell response in healthy pregnancies: However, the role of MDSC in patients suffering from miscarriage has not been studied. S100A8 level was analyzed by real time PCR, Western Blotting and ELISA. MDSC level was assessed by flow Cytometry and Immunostaining in blood and endometrial decidua respectively. Activation of T-cells was determined by MTT proliferation and IL-2 ELISA assays. The miscarriage patients harbor increased level of S100A8 and reduced level of functionally suppressive MDSC in blood and endometrium as compared to healthy control women with successful pregnancies. These results suggest S100A8 and MDSC are inversely co-related. MDSC level regulates maternal tolerance in healthy pregnancies and that drug inducing MDSC could have therapeutic implication in the miscarriage patients.