7^th Asia-Pacific Biotech Congress

Biography

Biography: P. Anitha

Abstract

Andrographis paniculata L., a member of Acanthaceae is a highly promising potential medicinal plant used worldwide for its wide range of pharmaceutical properties. The plant is used in traditional Chinese medicine (TCM), Ayurveda, Unani and Siddha systems of medicine. It contains Andrographolide esters which has got paramount importance in pharmaceutical field. Genetic analysis of normal, regenerated and Arbuscular Mycorrhiza Fungi (AMF) treated plants were performed using Amplified Fragment Length Polymorphism (AFLP) markers to facilitate reasoned scientific decisions on its management, conservation and for selective breeding programme. Normal and tissue cultured plants were treated with Glomus mosseae and G. fasciculatum to study the effect of symbiosis on the growth performance of both normal and tissue cultured plants. Analyses of morphological characters of tissue culture plants raised through nodal cultures and VAM treated plants have revealed phenotypic variations which prompted us to analyze the six samples through the molecular markers. AFLP markers were selected because of their widest application in analyses of genetic variations within the populations. Combinations of EcoR-I / Mse-I and Pst-I/ Mse-I were used for double digestion of the genomic DNA. 15 primer combinations were tried, out of which 6 combinations (Pst-I/ Mse-I) gave best results with bright and clear bands. The total number of bands produced for normal, regenerated and AMF treated plants were 641. Of these 495 bands was polymorphic indicating 77.2% of polymorphism. Analysis of molecular variance (AMOVA) revealed statistically significant differences between normal, tissue cultured and AMF treated plants. Based on this data binary matrix was prepared, dendrogram was constructed using  NTSYS-PC version-2  and UPGMA.