M Krishnan
Bharathidasan University, India
Title: Molecular characterization of vitellogenin receptor from Bombyx mori and Spodoptera litura: As a tool for pest management
Biography
Biography: M Krishnan
Abstract
In the everlasting races against the pests of agricultural importance, it is essential to develop a novel strategy for pest control. For this a thorough understanding of biological system of the insect pest at the cellular and molecular levels is needed. For instance, the recent trend involves the identification and characterization of physiologically and metabolically important proteins like insect vitellogenin, vitellogenin receptor, storage protein and apolipoporin which can be subjected to various refinements and manipulations for exploitation in pest management, in a target specific and eco-friendly manner. In the present investigation, we have studied the vitellogenin and vitellogenin receptor interaction. The N-terminal Ligand Binding Domain (LBD) of vitellogenin receptor is responsible for the transportation of vital protein (Vitellogenin) to the developing oocyte through receptor mediated endocytosis pathway. Ligand binding domain (LBD) sequence of B. mori noted to be completely different with that of Spodoptera litura though these two insects are coming under the lepidopteran group.The motif in the LBD were also species specific. VgR LBD from B. mori and S. litura were isolated and cloned in pET30a+ vector for over expression for structural studies. Since both insect with same order but LBD differences have pave way for understanding and implementing a new approach for species specific target drug development to inhibit the binding of Vg to the receptor specific to insect pest Spodoptera litura without affecting non targeted system.