7^th Asia-Pacific Biotech Congress

Biography

Biography: D.Leelavathi

Abstract

Lavandula angustifolia L. is a perennial shrub, belonging to the family Lamiaceae, cultivated for its leaves for extraction of essential oil. Oil is used in flavor, perfumery and pharmaceutical industries. In order to meet the growing demand of its oil and herbage, in vitro techniques are being used as alternative method for large scale multiplication and conservation. In the present investigation, in vitro apical bud explants were cultured on MS basal medium supplemented with BAP (8.88 µM) and NAA (2.68 µM) to induce multiple shoots. In vitro root explants were cultured on MS basal medium supplemented with BA (8.88 µM) and NAA (5.36 µM) to induce callus which was subcultured on same medium to obtain profuse callus. Callus was later cultured on shoot regenerating medium, MS supplemented with BAP (4.44 µM), KN (4.64 µM) and NAA (2.68 µM) to produce multiple shoots. Well developed multiple shoots developed roots on MS medium supplemented with BA (8.88 µM) and NAA (2.68 µM) and IBA (4.92 µM). The hardened, regenerated plants were acclimatized which were transferred to soil with 80% - 90% survival frequency. In vitro and in vivo leaves were subjected to phytochemical analysis for the determination of principle component. The in vitro apical buds were used for synthetic seed production using Sodium alginate and Calcium chloride as matrix and complexing agent for encapsulation. Hardened multiple shoots obtained from apical bud, root callus and synthetic seeds serve as a source of ex-situ conservation. The percentage of Linalool was found to be more in in vivo leaf when compared to in vitro propagules.