Hattem M Mekky
University of Alexandria Egypt
Title: Biological activity of elicited Echinacea purpurea suspension cultures
Biography
Biography: Hattem M Mekky
Abstract
Chitosan, phenyl alanine (Phe) and methyl jasmonate (MeJA) significantly increased the production of polyphenolics in suspension cultures of E. purpurea initiated on MS supplemented with 1.5 mg/L BA and 0.5 mg/L NAA. However, cultures elicited with 4 mM copper sulphate (CuSO4) possessed the highest EC100 in cytotoxicity experiments. Furthermore, Phe, MeJA, CuSO4 and chitosan significantly increased ABTS scavenging properties. Additionally, MeJA and Phe extracts stimulates the phagocytic and yeast intracellular killing activities of PMN and Finally, the anti-inflammatory properties of MeJA elicited cultures were the highest and were displayed by its strongest nitric oxide scavenging activity with an EC50 6.3 µg/ml and the most powerful inhibition of lymph proliferative activity induced by lipopolysaccharides with an EC50 4.5 µg/ml.
