8^th Euro Biotechnology Congress

Biography

Biography: Nasirzadeh Keyvan

Abstract

Silica-alginate  nanocomposites  were prepared by reinforcing the alginate beads with sili ca nanoparticles and used for microbial cell culture. Nonporous silica nanoparticl es (NSNs) and mesoporous silica nanoparticles (MSNs) were prepared and then amine-functionalized with N-(2-aminoethyl)-3-aminopropyl trimethoxy-silane (EDS) to prepare positively charged amine-n anoparticles. Different concentration of the prepared unfunctionalized and amine-functionalized silica nanoparticles were utilized to prepare reinforced alginate beads. Mechanical stability of the prepared beads was investigated and the Young's modulus values were calculated. DH5α cell were entrapped in the prepared beads and then their growth was studied. In addition, the toxicity of the silica particles on the growth of the cells was also studied. Young's modulus values of the beads reinforced with all the silica nanoparticles were markedly improved in comparison to that of the beads without any silica  nanoparticles . The Young's modulus values of the beads reinforced with amine-functionalized MSNs was measured to be app roximately 4.5 folds higher than that for the bare alginate beads. The existence of silica nanoparticles in the alginate beads not only increases their mechanical stability but also decreases the  leakage of cells from the beads. In addition, the results showed that the various silica nanoparticles prepared in this work does not show any significant effects on viability of the encapsulated cells. Finally, the superior properties of the prepared nanocomposite beads recommend them as a suitable cell culture system for microbial cells' cultivation for various applications.