Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 15th Asia-Pacific Biotechnology Congress Melbourne Australia.

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Scientific Program Day 1
Scientific Program Day 2

Day 2 :

Keynote Forum

Petr Maly,

BIOCEV Research Center, Czech Republic

Keynote: Targeting human IL-17 receptor by ABD-derived protein binders as a non-immunoglobulin alternative for modulation of Th-17-dependent pro-inflammatory response

Time : 9:30-10:30

Conference Series Asia Pacific Biotech Congress 2017 International Conference Keynote Speaker Petr Maly, photo
Biography:

Petr Maly is head of Laboratory of Ligand Engineering at the Institute of Biotechnology CAS in Vestec, Czech Republic. He studied at Department of Biochemistry, Faculty of Science, Charles University in Prague, Czech Republic (1980-1985) and completed doctorate at the Institute of Molecular Genetics CAS (IMG) in Prague. He spent postdoctoral fellowship (1992-1995) at Department of Pathology and Howard Hughes Medical Institute, The University of Michigan Medical School, Ann Arbor, USA. Since 1998 to 2005 he was a research group leader at the IMG in Prague and also worked at Department of Biochemistry and Molecular Biology, College of Medicine, University of Oklahoma, USA. He also was participating investigator of Consortium for Functional Glycomics (USA, 2001 2008) and Member of Editorial Board (2001-2005) and Editor (since 2003) of the Czech journal “Biologicke listy” (Biological Letters). Since 2008, he has been working on the development of combinatorial protein libraries derived from small protein scaffolds and construction of novel high-affinity protein binders with therapeutic and diagnostic potential.

Abstract:

Interleukin 17 (IL-17) and its cognate receptor (IL-17RA) play a crucial role in Th17 cell-mediated pro-inflammatory pathway and pathogenesis of several autoimmune disorders including psoriasis. Psoriasis is a chronic inflammatory skin disease with prevalence up to 3% worldwide and it is characterized by hyperplasia of the epidermis, infiltration of leukocytes into both dermis and epidermis, and dilation and growth of blood vessels. IL-17 is mainly produced by Th-17 helper cells and, via binding to its receptor, mediates IL-17-driven cell signaling in keratinocytes. Recently we have demonstrated that small protein binders derived from albumin binding domain (ABD) scaffold can serve as useful diagnostic tools (Ahmad et al. 2012, Sipova et al. 2012, Mareckova et al. 2015) as well as efficient blockers of IL-23/IL-23 receptor interaction and modulators of IL-23/Th-17 axis on primary human leukocytes (Krizova et al. 2017, Kuchar et al. 2014). In this work we generated a collection of novel protein binders targeting human IL-17RA, called ARS ligands, using selection from highly complex ABD-derived
combinatorial library in combination with ribosome display. From the group of 67 analysed ABD variants, 7 different sequence families were identified. Representatives of these groups competed with human IL-17A for binding to recombinant IL-17RA as well as to IL-17RA-IgG chimera, as tested in ELISA. Five ARS variants bind to IL-17RA-expressing THP-1 and Raji cells, as tested by flow cytometry. The four variants exhibited high-affinity binding in nanomolar range to human HaCAT cells, as measured using Ligand Tracer Green Line system. Thus, we identified several ARS inhibitory variants that will be further tested for their immunosuppressive function as novel IL-17RA antagonists.

  • Plant Biotechnology/Medical biotechnology/Pharmaceutical Biotechnology/
Location: Hampton Room

Chair

Abul Mandal

Session Introduction

Soraya Siabani

Kermanshah University of Medical Sciences, Iran

Title: Histopathology criteria suggesting microsatellite instability in colorectal cancer in Iranian patients
Speaker
Biography:

Soraya Siabani is an Associate Professor at Kermanshah University of Medical Sciences, Iran and an External Supervisor at University Technology Sydney, Australia. She has received her PhD in Public Health from the University of Sydney, Australia. She also has a Medical Doctorate from KUMS, Iran. Her research focuses on health promotion and epidemiology of chronic diseases.

Abstract:

Background & Aim: Optimization of the differentiation medium through using autologous factors such as PRP is of great consideration, but due to the complex, variable and undefined composition of PRP on one hand and lack of control over the absolute regulatory mechanisms in in vitro conditions or disrupted and different mechanisms in diseased tissue microenvironments in in vivo conditions on the other hand, it is complicated and rather unpredictable to get the desired effects of PRP making it inevitable to monitor the possible pathologic or undesired differentiation pathways and therapeutic effects of PRP. Therefore, in this study the probable potential of PRP on inducing calcification, inflammation and angiogenesis in chondrogenically differentiated cells was investigated.
Methods: The expressions of chondrogenic, inflammatory, osteogenic and angiogenic markers from TGF or PRP-treated cells during chondrogenic differentiation of human adipose-derived stem cells (ADSCs) was evaluated. Expressions of Collagen II (Col II), Aggrecan, Sox9 and Runx2 were quantified using q-RT PCR. Expression of Col II and X was investigated by immunocytochemistry as well. Glycosaminoglycans (GAGs) production was also determined by GAG assay. Possible angiogenic/inflammatory potential was determined by quantitatively measuring the secreted VEGF, TNF and phosphorylated VEGFR2 via ELISA. In addition, the calcification of the construct was monitored by measuring ALP activity and calcium deposition.

Results: Our data showed that PRP positively induced chondrogenesis; meanwhile the secretion of angiogenic and inflammatory markers was decreased. VEGFR2 phosphorylation and ALP activity had a decreasing trend,  ut tissue mineralization was enhanced upon treating with PRP.

Conclusions: Although reduction in inflammatory/angiogenic potential of the chondrogenically differentiated constructs highlights the superior effectiveness of PRP in comparison to TGF for chondrogenic differentiation, yet further improvement of the PRP-based chondrogenic differentiation media is required to inhibit the production of angiogenic/inflammatory markers, calcification and the release of synthesized GAG out of the construct.

Kang Bo Shim

National Institute of Crop Science, South Korea

Title: Effect of green manure crops treatments on the soil conditions and corn yield production
Speaker
Biography:

Kang Bo Shim has completed his PhD from Seoul National University. He is a Senior Researcher of Crop Cultivation & Environment Research Division, National Institute of Crop Science, Rural Development Administration at South Korea. He has published more than 20 papers.

Abstract:

This study was conducted to find optimum mixed sowing ratio of green manure crops to improve the chemical soil condition as well as to increase the crop yield potential which will foster the utilization of green manure crops in the upland field in view of environment friendly agriculture. According to the study, the mixed ratio, 50:50, of hairy vetch and green barley showed highest nitrogen production yield, 17. 2kg per 10a, in the soil due to the relatively higher organic nitrogen supply from the hairy vetch plant as well as nitrogen fixation from the air rather than other mixed ratio. Otherwise, other soil chemical conditions, such as pH, EC, Av. P2O3 and exchangeable cations, showed different results depending on the mixed sowing ratio of green manure crops. In case of pH, the mixed ratio, 100:0, treatment showed to increase to 7.0 from 6.0 of control (chemical fertilizer treatment). Available P2O3 amount showed highest at the mixed ratio, 100:0, treatment as 453 mg/kg. We also conducted the experiment to compare the effect of the mixed sowing treatment of green manure crops on the production of corn cultivated as a succeeding plant of green manure crops. The mixed ratio, 50:50, of hairy vetch and green barley treatment showed highest yield potential of corn as 153 kg per 10a in seed weight which is due to the relatively higher organic nitrogen supply from the hairy vetch plant as well as nitrogen fixation from the air rather than other mixed ratio. In the mixed sowing treatment of hairy vetch 100 and barley 0 ratio, the corn production showed 148 kg per 10a which is 5 kg lower than that of hairy vetch 50 and barley 50 ratio, but showed statistically no difference between those two treatment Therefore, we found that green manure crops, such as hairy vetch, green barley and rye were very effective crops to improve the soil fertility and gave the positive effect to the crop cultivation and, in turn, increased the yield potential.

Arezou Pakfar

Science and Research Branch of Islamic Azad University, Iran

Title: Expressions of pathological markers in PRP based chondrogenic differentiation of human adipose derived stem cells
Speaker
Biography:

Arezou Pakfar was graduated from Science and Research Branch of Islamic Azad University in Cellular and Molecular Biology. She has been working in Stem Cell
Technology Research Center since 2013.

Abstract:

Background & Aim: Optimization of the differentiation medium through using autologous factors such as PRP is of great consideration, but due to the complex, variable and undefined composition of PRP on one hand and lack of control over the absolute regulatory mechanisms in in vitro conditions or disrupted and different mechanisms in diseased tissue microenvironments in in vivo conditions on the other hand, it is complicated and rather unpredictable to get the desired effects of PRP making it inevitable to monitor the possible pathologic or undesired differentiation pathways and therapeutic effects of PRP. Therefore, in this study the probable potential of PRP on inducing calcification, inflammation and angiogenesis in chondrogenically differentiated cells was investigated.
Methods: The expressions of chondrogenic, inflammatory, osteogenic and angiogenic markers from TGF or PRP-treated cells during chondrogenic differentiation of human adipose-derived stem cells (ADSCs) was evaluated. Expressions of Collagen II (Col II), Aggrecan, Sox9 and Runx2 were quantified using q-RT PCR. Expression of Col II and X was investigated by immunocytochemistry as well. Glycosaminoglycans (GAGs) production was also determined by GAG assay. Possible angiogenic/inflammatory potential was determined by quantitatively measuring the secreted VEGF, TNF and phosphorylated VEGFR2 via ELISA. In addition, the calcification of the construct was monitored by measuring ALP activity and calcium deposition.
Results: Our data showed that PRP positively induced chondrogenesis; meanwhile the secretion of angiogenic and inflammatory markers was decreased. VEGFR2 phosphorylation and ALP activity had a decreasing trend, but tissue mineralization was enhanced upon treating with PRP.
Conclusions: Although reduction in inflammatory/angiogenic potential of the chondrogenically differentiated constructs highlights the superior effectiveness of PRP in comparison to TGF for chondrogenic differentiation, yet further improvement of the PRP-based chondrogenic differentiation media is required to inhibit the production of angiogenic/inflammatory markers, calcification and the release of synthesized GAG out of the construct.

Tom Levy

Ben-Gurion University, Israel

Title: Novel biotechnology for all-female prawn aquaculture by a single injection of hypertrophied androgenic gland cells
Speaker
Biography:

Tom Levy is currently pursuing his PhD studies at Ben-Gurion University, Israel. He is studying the controlling mechanism behind sexual differentiation in crustaceans in order to shed light on crustacean's reproductive physiology which will also pave the way for the establishment of sustainable biotechnological tools to achieve monosex populations. With his colleagues from Ben-Gurion University and Enzootic Ltd., he has recently published a paper on a novel biotechnology to achieve all-female aquaculture of the giant freshwater prawn.

Abstract:

Crustacean monosex aquaculture is advantageous since most species exhibit dimorphic size variation between males and females. In the case of the freshwater prawn, Macrobrachium rosenbergii, intensification of mixed cultures encounters difficulties due to the complex social structure, in which large dominant males are territorial and inhibit the growth performance of the rest of the population. Females on the other hand are less aggressive, less territorial and are suggested to exhibit a relatively homogenous size which does not require selective harvests during or in the end of the grow-out period. In the present study, a novel biotechnology to achieve M. rosenbergii monosex female populations was developed. Our biotechnology includes the following three steps: (1) a single injection of suspended hypertrophied androgenic gland cells (hAG cells) induced fully functional sex-reversal of females into ‘Neo-Males’ bearing the feminine WZ genotype; (2) crossing neo-males with normal females (WZ), yielded progeny containing ~25% WW females as validated by specific DNA sex markers and (3) WW females were crossed with normal males (ZZ) and gave rise to all-female progenies. This biotechnology enabled the first ever large-scale field study showing better performance for all-female prawn culture than mixed culture in all parameters including: Higher survival rate, higher yield per hectare and uniformity of marketable product size. Additionally, no males or egg carrying females were found while examining the harvest of tenth of thousands animals in the all-female ponds. The latter result points at the reliability of our technology to achieve all-female populations and its sustainability with respect to the non-reproductive nature of the monosex culture securing both protection of proprietary elite lines and avoiding environmental invasions by escapees.

Jian Xing Wu

National Synchrotron Radiation Research Center, Taiwan

Title: An FPGA‐based chaos synchronisation classifier for estimation of peripheral vascular occlusion using photoplethysmography
Speaker
Biography:

Jian-Xing Wu was born in 1985. He received the B.S. degree in Electrical Engineering from the Southern Taiwan University of Science and Technology, Tainan, Taiwan, in 2007, the M. S. degree in Electrical Engineering from the Southern Taiwan University of Science and Technology, Tainan city, Taiwan, in 2009 and the Ph.D. degree in Biomedical Engineering from National Cheng Kung University, Tainan city, Taiwan, in 2014. Currently, he is a postdoctoral research fellow of Experimental Technique Group, National Synchrotron Radiation Research Center, where has been since 2014. His research interests include biomedical signal processing, medical ultrasound and medical device design, X-ray microscopy.

Abstract:

This study proposes a method for the estimation of peripheral vascular occlusion (PVO) in diabetic foot using a sprott chaos synchronisation (SCS) classifier with the wolf pack search (WPS) algorithm. The longterm presence of elevated blood sugar levels commonly results in peripheral neuropathy, peripheral vascular disease, nephropathy, and retinopathy in patients with Type 2 diabetes mellitus. Patients with PVO disease have decreased walking capability and life quality in diabetes mellitus and poor peripheral circulation of PVO causes morbidity like infection and amputation of the legs or feet of diabetics. This progressively vascular occlusion is often ignored by the patients and primary care physicians in early stage. Therefore, a reliable method of diagnostic assistance is crucial for early diagnosis and monitoring of PVO and prevention of amputation. Photoplethysmography (PPG) is a non-invasive technique for detecting blood volume changes in peripheral vascular bed. Literature indicates that the pulse transit time increases and waveform shape changes increase in PPG of the vascular occlusion. PPG pulses of feet gradually become asynchronous due to the different speed of deteriorating patency and collateral circulation in the peripheral arteries. We utilized synchronizing chaotification to compare the bilateral similarity and asymmetry of PPG signals, and applied SCS to estimate three degrees of PVO. Among 30 subjects tested, this classification technique could recognize various butterfly motion patterns representing severities successfully including normal condition, lower-degree disease, and higher-degree disease. The proposed method has potential for providing diagnostic assistance for PVO of diabetics and other high-risk populations, with efficiency and higher accuracy.